Journal: Journal of the Chinese Medical Association : JCMA

Article Title: TRIM72 mediates lung epithelial cell death upon hyperoxia exposure

doi: 10.1097/JCMA.0000000000000413

Figure Lengend Snippet: Immunohistochemistry results (A) and Western blotting results and quantitative data (B, C) for TRIM72 expression in 7-day-old and 14-day-old rats in the normoxia and O 2 -enriched groups. TRIM72 immunoreactivity was observed in endothelial cells (black arrow), superior surface of bronchial epithelial cells (red arrow), and alveolar type I (short black arrow) and II cells (short red arrow). The normoxia group showed faint immunoreactivity in bronchial epithelial cells (red arrow) and alveolar type I (short black arrow) and II cells (short red arrow). The rats in the O 2 -enriched group exhibited significantly higher TRIM72 expression than those in the normoxia group on postnatal days 7 and 14. * p < 0.05.

Article Snippet: RLE-6TN, a rat alveolar type II epithelial cell line, and A549, a human lung carcinoma epithelial cell line (ATCC, Manassas, VA, USA), were maintained in an F-12 medium in 75-cm 2 tissue culture flasks at 37.8°C in 5% CO 2 and 95% air.

Techniques: Immunohistochemistry, Western Blot, Expressing

Journal:

Article Title: Effects of p21 Cip1/Waf1 at Both the G 1 /S and the G 2 /M Cell Cycle Transitions: pRb Is a Critical Determinant in Blocking DNA Replication and in Preventing Endoreduplication

doi:

Figure Lengend Snippet: Cell lines used in this study showing which were stably transfected with the tetracycline system and which were transduced with adenoviruses for p21 or p27

Article Snippet: A549 human lung carcinoma cells and Kb human epidermoid carcinoma cells (Duncan Walker, Glaxo Wellcome), WI38 human diploid fibroblasts (American Type Culture Collection), and HaCat cells (N. Fusenig, Heidelberg, Germany) were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in a 37°C incubator with 5% CO 2 .

Techniques: Stable Transfection, Transfection, Transduction

Journal:

Article Title: Effects of p21 Cip1/Waf1 at Both the G 1 /S and the G 2 /M Cell Cycle Transitions: pRb Is a Critical Determinant in Blocking DNA Replication and in Preventing Endoreduplication

doi:

Figure Lengend Snippet: G 1 accumulation correlates with the presence of functional Rb and inversely correlates with endoreduplication

Article Snippet: A549 human lung carcinoma cells and Kb human epidermoid carcinoma cells (Duncan Walker, Glaxo Wellcome), WI38 human diploid fibroblasts (American Type Culture Collection), and HaCat cells (N. Fusenig, Heidelberg, Germany) were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in a 37°C incubator with 5% CO 2 .

Techniques: Functional Assay